Main Article Content
Abstract
Cilegon City, Banten Province, Indonesia, generates abundant organic waste in the form of marine fish viscera (MFV) from fish-cleaning activities in traditional markets, which remains largely underutilized. This study aimed to evaluate the potential of MFV as an alternative bacterial starter for biogas production through the co-digestion of water hyacinth (KIM) and corn husk (KJ) biomass. Prior to digestion, KIM and KJ were physically pretreated to a particle size of 50–60 mesh, then chemically pretreated with 0.1 M NaOH. The pretreated biomass was then oven-dried at 60 °C for 24 h. Various KIM: KJ mass ratios were investigated, including 10:0, 8:2, 6:4, 4:6, 2:8, and 0:10, with a constant total solids (TS) content of 10 g. In addition, the biomass-to-MFV starter ratios were varied to 1:0.5, 1:1, 1:1.5, and 1:2. Anaerobic batch fermentation was conducted at an initial pH of 8, with 160 mL of distilled water added per 10 g TS. Methane content was analyzed using a Shimadzu GC-8A equipped with a thermal conductivity detector (TCD) at the National Research and Innovation Agency (BRIN), Indonesia. Biogas volume was measured at two-day intervals over a 50-day fermentation period. The results demonstrated that the optimum biomass composition was achieved at a KIM:KJ ratio of 4:6, yielding a biogas production of 65 mL g⁻¹ TS. The optimum biomass-to-starter ratio of 1:1 resulted in a total biogas volume of 986 mL, a biogas yield of 99 mL g⁻¹ TS, and an organic matter conversion efficiency of 65% after 50 days of fermentation, with methane content reaching 60% (v/v). This study introduces a novel approach by demonstrating, for the first time, the use of marine fish viscera as a bacterial starter in the co-digestion of KIM and KJ, highlighting its significant potential to enhance biogas yield while valorizing marine processing waste.
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References
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- Ritonga, A., & Masrukhi. (2019). Optimization Of Methana Content (CH4) Biogas of Cow Dug Using Various Types of Adsorben. 10, 8–17.
- Safitri, W., Novalina, S. A., Apriandi, A., Hasil Perikanan, T., & Ilmu Kelautan dan Perikanan, F. (2023). Manufacture of Local Microorganisms (MOL) From Fish Offal. Marinade, 06(010), 1–7.
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- Sulthan Nafis Nabila dan Okik Hendriyanto C. (2021). Produksi Biogas Dari Kombinasi Kotoran Kambing dan Limbah Ikan Dalam Biodigester Anaerob Sulthan. 2, 88–95.
- Syaichurrozi, I., Suhendi, E., Kustiningsih, I., & Nurulshani, S. (2026). Bioresource Technology Reports Enhancement of biogas production through anaerobic co-digestion of tofu wastewater and cassava starch wastewater in Indonesia. Bioresource Technology Reports, 33 (December 2025), 102557. https://doi.org/10.1016/j.biteb.2026.102557
- Syaichurrozi, I., Suhirman, S., & Hidayat, T. (2018). Effect of initial pH on anaerobic co-digestion of Salvinia molesta and rice straw for biogas production and kinetics. Biocatalysis and Agricultural Biotechnology, 16, 594–603. https://doi.org/10.1016/j.bcab.2018.10.007
- Syaichurrozi, I., Suhirman, S., & Hidayat, T. (2021). Effect of Substrate/Water Ratio on Biogas Production from the Mixture Substrate of Rice Straw and Salvinia molesta. Jurnal Riset Teknologi Pencegahan Pencemaran Industri, 12(2), 45–55. https://doi.org/10.21771/jrtppi.2021.v12.no2.p45-55
- Vivekanand, V., Mulat, D. G., Eijsink, V. G. H., & Horn, S. J. (2018). Synergistic effects of anaerobic co-digestion of whey, manure and fish ensilage. Bioresource Technology, 249, 35–41. https://doi.org/10.1016/j.biortech.2017.09.169
References
Anggakara, P., Sudarno, & Wardhana, I. W. (2013). Pengaruh Pengenceran dan Pengadukan terhadap Produksi Biogas pada Limbah Industri Kecil Pengasapan Ikan dengan Menggunakan Ekstrak Rumen Sapi Sebagai Starter. Teknik Lingkungan, 1(1), 1–8.
Budiyono, B., Matin, H. H. A., Yasmin, I. Y., & Priogo, I. S. (2023). Effect of Pretreatment and C/N Ratio in Anaerobic Digestion on Biogas Production from Coffee Grounds and Rice Husk Mixtures. International Journal of Renewable Energy Development, 12(1), 209–215. https://doi.org/10.14710/ijred.2023.49298
Budiyono, B., Syaichurrozi, I., Suhirman, S., Hidayat, T., & Jayanudin, J. (2021). Experiment and modeling to evaluate the effect of total solid on biogas production from the anaerobic co-digestion of Tofu liquid waste and rice straw. Polish Journal of Environmental Studies, 30(4), 3489–3496. https://doi.org/10.15244/pjoes/127277
Damayanti, S. I., Hakim, R. A., Anggraeni, A., & Saputri, Y. (2024). Effect of Codigestion of Rice Straw , Fish Meal , and Cow Manure on Biogas Production and Quality of Solid Bioslurry Fertilizer. 13(4), 1272–1284.
Dwityaningsih, R., Rahayu, T. E. P. S., Handayani, M., & Mardiyana, M. (2024). Analisis Proses Pembentukan Biogas dari Campuran Limbah Ikan, Kotoran Sapi dan Eceng Gondok (Eichhornia Crassipes). Acropora: Jurnal Ilmu Kelautan Dan Perikanan Papua, 7(1), 44–51. https://doi.org/10.31957/acr.v7i1.3806
Engga Noer Wildan Efendi, Jumsurizal, J., & Amrizal, S. N. (2022). Pemanfaatan Limbah Jeroan Ikan Tongkol (Euthynnus Affinis) dan Rumput Laut Coklat (Sargassum Polycystum) Sebagai Pupuk Padat Terhadap Pertumbuhan Tanaman Sawi (Brassica juncea L.). Marinade, 5(01), 28–36. https://doi.org/10.31629/marinade.v5i01.4058
Evi Arianingsih, Irdha Mirdhayati, & Anwar Efendi Harahap. (2021). Kualitas Biogas Berbahan Feses Sapi dan Jerami Jagung (Zea mays L.) pada C/N Rasio dan Lama Fermentasi yang Berbeda. Jurnal Triton, 12(1), 58–67. https://doi.org/10.47687/jt.v12i1.155
Fariadi, H., Yulihartika, R. D., Azhari, D., & Saputra, J. (2024). Sosialisasi Limbah Tulang Ikan Sebagai Bahan Baku Pengolahan Produk Pangan Inovatif. Jurnal Dehasen Untuk Negeri, 3(1), 143–148. https://doi.org/10.37676/jdun.v3i1.5592
Garwan, R., Kusumaningrum, H. D., Nurhayati, T., & Lioe, H. N. (2022). Characterization of Skipjack Viscera as Initial Screening of Sources for Housefly Traps Musca domestica and Antibacterial. Jurnal Pengolahan Hasil Perikanan Indonesia, 25(1), 34–51. https://doi.org/10.17844/jphpi.v25i1.38555
I.R. Zulkarnaen, S., H. T., & Padang, Y. A. (2018). Pengaruh Rasio Karbon Dan Nitrogen (C/N Ratio) Pada Kotoran Sapi Terhadap Produksi Biogas dari Proses Anaerob. Dinamika Teknik Mesin, 1–16.
Irnawati, R., Surilayani, D., Susanto, A., Rahmawati, A., Munandar, A., Sari, R., & Nurdin, H. S. (2020). Analysis of Determining the Base Location of Anchovy Fisheries and its Marketing Distribution in The Banten Province. Jurnal Sosial Ekonomi Kelautan Dan Perikanan, 15(2), 159–168.
Matin, H. H. A., Syafrudin, & Nugraha, W. D. (2016). Pengaruh C/N Ratio Pada Produksi Biogas Dari Limbah Sekam Padi Dengan Metode Solid State Anaerobic Digestion (SS-AD). Jurnal Teknik Lingkungan, Vol. 5, No. 4 (2016), 5(1), 1–10.
Nabila, S. N., & Hendriyanto, O. (2021). Produksi Biogas Dari Kombinasi Kotoran Kambing. Volume 2 N, 1–8.
Perdana, D. A., Ebrianto, A. L., & Sari, T. I. (2013). Penggunaan Starter Envirosolve Dan Dari Bahan Baku Ampas Tahu. 19(1).
Purnamasari, E., Fanani, M., Anwar, &, & Harahap, E. (2024). Organic-C content, nitrogen content, final C/N ratio value, pH value, temperature, and biogas. Seminar Nasional Integrasi Pertanian Dan Peternakan, 2(1), 193–204. https://semnasfpp.uin-suska.ac.id/index.php/snipp
Ritonga, A., & Masrukhi. (2019). Optimization Of Methana Content (CH4) Biogas of Cow Dug Using Various Types of Adsorben. 10, 8–17.
Safitri, W., Novalina, S. A., Apriandi, A., Hasil Perikanan, T., & Ilmu Kelautan dan Perikanan, F. (2023). Manufacture of Local Microorganisms (MOL) From Fish Offal. Marinade, 06(010), 1–7.
Saputra, A. O., Mafruddin, M., Handono, S. D., & Riduan, M. (2023). Pengaruh Jenis Starter Terhadap Produktivitas dan Nilai Kalor Biogas Sampah Organik. Turbo : Jurnal Program Studi Teknik Mesin, 12(2). https://doi.org/10.24127/trb.v12i2.2894
Siddharth, Shesh Vijay Sharma, & V. K. Pandey. (2024). Isolation and Characterization of Microorganisms Involved in Biogas Production from Agricultural Waste. International Journal of Scientific Research in Science and Technology, 11(2), 718–730. https://doi.org/10.32628/ijsrst24112118
Suhirman, S. (2023). Uji Kemampuan Partikel KMnO4 Teremban Dalam Karbon Aktif Tempurung Kelapa Sawit Terhadap H2S Dalam Reaktor Biogas Unggun Tetap. UNISTEK, 10, 134–143. https://doi.org/10.33592/unistek.v10i2.3806
Sulthan Nafis Nabila dan Okik Hendriyanto C. (2021). Produksi Biogas Dari Kombinasi Kotoran Kambing dan Limbah Ikan Dalam Biodigester Anaerob Sulthan. 2, 88–95.
Syaichurrozi, I., Suhendi, E., Kustiningsih, I., & Nurulshani, S. (2026). Bioresource Technology Reports Enhancement of biogas production through anaerobic co-digestion of tofu wastewater and cassava starch wastewater in Indonesia. Bioresource Technology Reports, 33 (December 2025), 102557. https://doi.org/10.1016/j.biteb.2026.102557
Syaichurrozi, I., Suhirman, S., & Hidayat, T. (2018). Effect of initial pH on anaerobic co-digestion of Salvinia molesta and rice straw for biogas production and kinetics. Biocatalysis and Agricultural Biotechnology, 16, 594–603. https://doi.org/10.1016/j.bcab.2018.10.007
Syaichurrozi, I., Suhirman, S., & Hidayat, T. (2021). Effect of Substrate/Water Ratio on Biogas Production from the Mixture Substrate of Rice Straw and Salvinia molesta. Jurnal Riset Teknologi Pencegahan Pencemaran Industri, 12(2), 45–55. https://doi.org/10.21771/jrtppi.2021.v12.no2.p45-55
Vivekanand, V., Mulat, D. G., Eijsink, V. G. H., & Horn, S. J. (2018). Synergistic effects of anaerobic co-digestion of whey, manure and fish ensilage. Bioresource Technology, 249, 35–41. https://doi.org/10.1016/j.biortech.2017.09.169