Article Sidebar

Submitted
July 23, 2021
Published
July 23, 2021
Download
PDF
Statistic
Read Counter : 1172 Download : 88

Main Article Content

Abstract

ABSTRACT Premna serratifolia L. leaves contain flavonoids, saponins, polyphenols, and terpenoids, potentially act as sunscreens. The purpose of this study was to determine the flavonoid content and SPF (Sun Protection Factor) ethanol extract of Premna serratifolia L. leaves from Melawi Regency, West Kalimantan Province using UV-Vis spectrophotometer. The Premna serratifolia L. leaves were extracted by maceration using 70% ethanol solvent, then the filtrate obtained was concentrated with a rotary vacuum evaporator to obtain a thick extract. The extract was determined for its flavonoid content using UV-Vis spectrophotometer at a wavelength of 439 nm, and the SPF value was determined at a concentration of 25, 50, 75, and 100 ppm with a UV-Vis spectrophotometer at a wavelength of 290-320 nm. The SPF value was calculated and analyzed descriptively using the Mansur method. The result showed that the average flavonoid content of ethanol extract of Premna serratifolia L. leaves was 3.70±0.02% calculated as quercetin. The SPF EEDB values obtained from variations in concentrations of 25, 50, 75, and 100 ppm respectively were 19.95±0.02; 30.08±0.01; 34.58±0.14; and 38.28 ± 0.12, where the SPF value is included in the ultra protection category. Key words: Premna serratifolia L. leaves, flavonoid content, SPF, UV-Vis Spectrophotometer

Article Details

References

  1. Basset, J., R. C., Denney, G.H., Jeffrey, J., and Mendhom., 1994, Buku Ajar Vogel Kimia Analisis Kuantitatif Anorganik, EGC, Jakarta, 846-848.
  2. Depkes RI, 2000. Parameter Standar Umum Ekstrak Tumbuhan Obat, Direktorat Jendral Pengawasan Obat dan Makanan, Jakarta, 28, 30, 32, 35.
  3. Kurniati, R. I., 2013. Uji Aktivitas Antioksidan Fraksi Etanol Daun Buas-Buas (Premna cordifolia Linn.) dengan Metode DPPH (2,2–difenil–1–pikrilhidrazil). Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN, 3(1).
  4. Mansur, J.S, Breder, M. N. R, Mansur, M. C. A, Azulary, R.D. 1986, Determination of Sun Protection Factor for Spectrophotometry, An Bras Dermatol. 62:121-124.
  5. Palpon D, N., 2017, Penetapan Kadar Flavonoid Total Ekstrak Etanol Daun buas- buas (Premna foetida reinw. Ex blume). Karya Tulis Ilmiah. Akademi Farmasi Yarsi, Pontianak.
  6. Puspita, W, Sari, D.Y., dan Rahman I.R., 2020, Uji Aktivitas Antioksidan Ekstrak Etanol Daun Buas-buas (Premna serratifolia L.) Asal Kabupaten Melawi Provinsi Kalimantan Barat Dengan Metode DPPH, Jurnal Insan Farmasi Indonesia, 3(2), 405-412.
  7. Sari, A. K, 2015, Penetapan Kadar Polifenol Total, Flavonoid Total, Dan Uji Aktivitas Antioksidan Ekstrak Etanol Daun Sirsak (Annona muricata) Dari Jember Pada Ketinggian Tanah Yang Berbeda. Skripsi. Universitas Jember.
  8. Supratman, U., 2010, Elusidasi Struktur Senyawa Organik, Widya Padjajaran Bandung
  9. Vickery, A, 1984, Ekologi Hutan Indonesia. UGM Press. Jogjakarta.
  10. Wilkinson, J. B., dan Moore, R. J., 1982, Harry’s Cosmeticology 7th Ed, Chemical Publishing Company, New York.
  11. Wulandari, R., dan Utom, P. P., 2019, Skrinning Fitokimia dan Aktivitas Antioksidan Teh Herbal Daun Buas-buas (Premna cordifolia Roxb.). Jurnal Dinamika Penelitian Industri, 30(1) : 117- 122