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December 17, 2025
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May 1, 2026
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Abstract

Garlic (Allium sativum) is known to have antioxidant activity that is beneficial to health. Antioxidant activity is expressed in IC₅₀ value, which is the concentration of the extract that can reduce 50% of free radicals. This study aims to test the antioxidant activity of a gel formulated from garlic extract using the DPPH (2,2-diphenyl-1-pyrrylhydrazine) method. The garlic extract and garlic gel extract were tested to evaluate the % inhibition and IC₅₀ values. Garlic ethanol extract showed an IC50 value of 89,290, and garlic ethanol extract gel showed the % inhibition value of 87,30. The decrease in the IC₅₀ value indicates that the gel formulation provides significant antioxidant activity against DPPH free radicals. In conclusion, the ethanol extract of Allium sativum formulated into a gel showed antioxidant activity based on IC₅₀ and percentage of inhibition values obtained from the DPPH assay.

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References

  1. Amirna, N., Widiastuti, H., Trihadi, A., & Kisra, K. (2024). Kapasitas antioksidan dari ekstrak etanol bawang putih (Allium sativum) secara in vitro. Makassar Pharmaceutical Science Journal, 1(28), 251. https://journal.farmasi.umi.ac.id/index.php/mpsj
  2. Ansong, J. A., Asante, E., Johnson, R., Boakye-Gyasi, M. El, Kuntworbe, N., Owusu, F. W. A., & Ofori-Kwakye, K. (2023). Formulation and evaluation of herbal-based antiacne gel preparations. BioMed Research International, 2023. https://doi.org/10.1155/2023/7838299
  3. Ardiansyah, F. F., Latifa, M. F., & Rohmawati, L. (2023). Uji Sifat Fisik Sediaan Salep Luka Ekstrak Daun Katuk (Sauropus androgynus (L.) Merr.). Proceedings of Seminar Nasional Fisika (SNF) Unesa 2023. Universitas Negeri Surabaya.
  4. Boeing, H., Bechthold, A., Bub, A., Ellinger, S., Haller, D., Kroke, A., Leschik-Bonnet, E., Müller, M. J., Oberritter, H., Schulze, M., Stehle, P., & Watzl, B. (2012). Vegetables and fruit in the prevention of chronic diseases: A critical review. European Journal of Nutrition, 51(6), 637–663. https://doi.org/10.1007/s00394-012-0380-y
  5. Damayanti, R., Hasan, T. & Iqbal, M. (2024). Skrining fitokimia dan uji aktivitas antioksidan ekstrak etanol bawang putih (Allium sativum L.) dengan metode ABTS. Jurnal Novem Medika Farmasi, 3(1), pp. 11–20
  6. Farhan, M., Putriana, R. A., & Humaidi, F. (2023). Formulasi dan uji mutu fisik sediaan gel ekstrak bunga telang (Clitoria ternatea L.) sebagai antiseptik tangan. Jurnal Farmasi dan Herbal, 5. http://ejournal.delihusada.ac.id/index.php/JPFH
  7. Fatmawati, F., Santoso, R., Azhar, D. P., Desvita, N. A., & Fauziah, R. (2020). Uji penghambatan tirosinase masker gel peel off yang mengandung rumput laut (Euchema cotonii) dan whey kefir. Indonesia Natural Research Pharmaceutical Journal, 5(1), 94–104.
  8. Ghali, R., Limam, I., Kassrani, I., Araoud, M., Nouiri, E., Fennira, F. B., Abdelkarim, M., & Hedilli, A. (2025). LC-MS profiling and antioxidant, antifungal, and anticancer potentials of Tunisian Allium sativum L. extracts. PloS one, 20(6), e0325227. https://doi.org/10.1371/journal.pone.0325227
  9. Li, F., Li, Q., Wu, S., & Tan, Z. (2017). Salting-out extraction of allicin from garlic (Allium sativum L.) based on ethanol/ammonium sulfate in laboratory and pilot scale. Food chemistry, 217, 91–97. https://doi.org/10.1016/j.foodchem.2016.08.092
  10. Maharani, A. I., Riskierdi, F., Febriani, I., Kurnia, K. A., Rahman, N. A., Ilahi, N. F., & Farma, S. A. (2021). Peran antioksidan alami berbahan dasar pangan lokal dalam mencegah efek radikal bebas. Prosiding SEMNAS BIO 2021, 390–399.
  11. Mokoginta, R. V., Simbala, H. E. I., & Mansauda, K. L. R. (2020). Antioxidant activity test of ethanol extracts of dayak onion bulbs (Eleutherine americana Merr) with DPPH method (1,1-Diphenyl-2-Picrylhydrazyl). Pharmacon, 9(3).
  12. Molyneux, P. (2004). The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26(2), 211–219.
  13. Novia, I. K., Ikhda, N. H. S., & Farma, S. A. (2020). Formulasi dan uji mutu fisik sediaan gel ekstrak bekatul (Oryza sativa L.). Jurnal Farmasi Mitra Sehat Mandiri Sidoarjo.
  14. Nurkhasanah, Bachri, S. M., & Yuliani, S. (2023). Antioksidan dan stres oksidatif (G. A. Sabilla, Ed.; 1st ed.). UAD Press.
  15. Pandel, R., Poljšak, B., Godic, A., & Dahmane, R. (2013). Skin photoaging and the role of antioxidants in its prevention. ISRN Dermatology, 2013, 1–11. https://doi.org/10.1155/2013/930164
  16. Pramiastuti, O., Rejeki, D. S., & Febriani, V. (2021). Formulasi gel ekstrak bawang putih (Allium sativum L.) dengan kombinasi basis Carbopol dan Na-CMC. Parapemikir: Jurnal Ilmiah Farmasi, 10(2), 113. https://doi.org/10.30591/pjif.v10i2.2471
  17. Rashati, D., Falahi, A., Mikhania, Rohman, A. D., & Maulani, D. (2024). Formulasi dan uji stabilitas fisik sediaan gel ekstrak kulit buah pisang (Musa acuminata Colla) dengan variasi enhancer. Jurnal Ilmiah Farmasi Akademi Farmasi, 7(1), 2615–2756.
  18. Rinaldi, Fauziah, & Zakaria, N. (2021). Studi formulasi sediaan gel ekstrak etanol serai wangi (Cymbopogon nardus (L.) Randle) dengan basis HPMC. Jurnal Ilmiah Farmasi Simplisia, 1(1), 33–42.
  19. Romsiah, P., Putri, E. P., & Erjon. (2020). Perbandingan aktivitas antioksidan ekstrak bawang putih (Allium sativum L.), bawang putih tunggal (Allium sativum var. Solo garlic), dan black garlic dengan metode DPPH. Jurnal Ilmiah Bakti Farmasi, 5, 45–50.
  20. Sa’adah, F. D., Mirza, D. M., & Wulandari, D. N. (2024). Potensi antioksidan fraksi n-heksana daun ubi jalar ungu (Ipomoea batatas L.). Jurnal Kesehatan Islam: Islamic Health Journal, 13(2), 1–10. https://doi.org/10.33474/jki
  21. Shang, A., Cao, S. Y., Xu, X. Y., Gan, R. Y., Tang, G. Y., Corke, H., Mavumengwana, V., & Li, H. Bin. (2019). Bioactive compounds and biological functions of garlic (allium sativum L.). In Foods (Vol. 8, Issue 7). MDPI Multidisciplinary Digital Publishing Institute. https://doi.org/10.3390/foods8070246
  22. Sumati Popala, J., Mongie, J., Tulandi, S. S., & Montolalu, F. (2022). Aktivitas antioksidan ekstrak daun pining bawang (Horntedtia alliacea). The Tropical Journal of Biopharmaceutical, 5(1), 18–28.
  23. Suryani, A. E. P. P., & Agustyiani, P. (2017). Formulasi dan uji stabilitas sediaan gel ekstrak terpurifikasi daun paliasa (Kleinhovia hospita L.). Pharmacon: Jurnal Ilmiah Farmasi UNSRAT, 6(3).
  24. Thomas, N. A., Tungadi, R., Hiola, F., & Latif, M. S. (2023). Pengaruh konsentrasi Carbopol 940 sebagai gelling agent terhadap stabilitas fisik sediaan gel lidah buaya (Aloe vera). Indonesian Journal of Pharmaceutical Education, 3(2). https://doi.org/10.37311/ijpe.v3i2.18050
  25. Tutik, F., Junova, H., & Anatasia, I. (2021). Formulasi sediaan gel moisturizer anti-aging ekstrak kulit bawang merah (Allium cepa L.) sebagai antioksidan. Jurnal Farmasi Malahayati, 4(1).
  26. Verma, T., Aggarwal, A., Dey, P., Chauhan, A. K., Rashid, S., Chen, K. T., & Sharma, R. (2023). Medicinal and therapeutic properties of garlic, garlic essential oil, and garlic-based snack food: An updated review. Frontiers in Nutrition, 10. https://doi.org/10.3389/fnut.2023.1120377
  27. Wakhidah, L., & Anggarani, M. A. (2021). Analisis senyawa bioaktif dan aktivitas antioksidan ekstrak bawang putih (allium sativum L.) probolinggo. Unesa Journal of Chemistry, 10(3), 356-366.
  28. Yulita, N. N. T. S., Edy, H. J., & Siampa, J. P. (2019). Formulasi sediaan gel ekstrak etanol daun kaliandra (Calliandra surinamensis Benth) sebagai antibakteri. Pharmacon: Program Studi Farmasi, FMIPA, Universitas Sam Ratulangi, 8(4), 801–808.
  29. Yuniarto, K., Muvianto, M. O., & Studi Teknik Pertanian, F. T. P. A. (2021). Aplikasi ultrasound assisted extraction untuk produksi minyak bawang putih varietas lokal. Jurnal Teknologi Pertanian, 22(3).