The In Vitro Evaluation of Cholesterol-Lowering Activity of Acetyleugenol Synthesized via Esterification of Eugenol

Abstract

: Hypercholesterolaemia is a significant risk factor for cardiovascular disease that necessitates alternative therapies instead of statins due to their adverse effects.  Eugenol, a phenolic molecule that has the ability to lower cholesterol levels, is found in clove oil, which is derived from Syzygium aromaticum.  The chemical stability and biological activity of its derivative, acetyleugenol, are both significantly higher.  The purpose of this research was to determine whether or not acetyleugenol, which was produced by esterifying eugenol with acetic anhydride, have the ability to be effective in decreasing cholesterol levels.  In order to determine the anticholesterol activity, UV-Vis spectrophotometry with the Liebermann-Burchard method was utilised at a range of concentrations (50, 75, 100, 125, and 150 ppm).  The EC50 value was equal to 94.413 ppm, and the maximum cholesterol decrease was 27.97% when the concentration was 150 ppm.  Considering these data, it appears that acetyleugenol possesses a considerable amount of potential as a natural cholesterol-lowering medication.